Cytological studies of grapevine leafroll infected tissue: Further evidence of viroid etiology and improvement of diagnosis
DOI:
https://doi.org/10.5073/vitis.1987.26.37-41Keywords:
leafroll, disease, cytology, symptomatology, selection, plant protectionAbstract
In the cells of leafroll-infected grapevines mainly two kinds of inclusions were found, similar to those as previously described in citrus exocortis viroid-infected Gynura aurantiaca D. C. cells: cytoplasmic granular inclusions (presumably viroid derivatives or aggregations) and protein circular inclusions occupying the infected nucleoplasms. The observation of these inclusions could substitute the traditional leafroll indexing technique. The duration of this simple cytological diagnosis is approximately 30 min; it could be an important aid for the programs of sanitary selection or sanitation of the grapevine. The study confirms cytologically a recent paper based on b.iochemical techniques, contributing further evidence for the viroid etiology of this disease.Downloads
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