Der Einfluß von Wachstumsinhibitoren auf die Langzeitlagerung von <i>in-vitro</i>-Kulturen der Rebe

Abstract

1. The effect of Cycocel (CCC) and Alar (B 995) on the growth of in vitro cultures of grapevine was tested under temperatures of +25, +8 and +3 °C. Both inhibitors were applied to the sterilized medium.
2. Alar caused in rather low concentrations (100 ppm) such a strong growth inhibition that subculturing became difficult. Moreover, the once induced inhibition was maintained in the two subsequent passages after Alar application.
3. CCC at concentrations of 500 to 1000 ppm reduced the length of the internodes of all cultivars tested to an extent of 30-40 % and the number of nodes/pla ntlet to 20-30 %, but delayed subculturing from 2-3 months to 5-7 months. An after-effect of CCC on subcultured plants was not observed.
4. CCC-treated plantlets had sma ller and darker green leaves. Callus formation at the base of the explants was stimulatecl, whereas root growth was either unaffectecl or s lightly enhanced.
5. CCC (750 ppm) prolonged vitality of the explants during a long- term storage at +8 °C.
6. CCC-treated plantlets (750 ppm) survivecl after a storage period of 10 months at a recluced stor age t emperature of +3 °C without any loss of vitality, whereas untreated plantlets did not survive.
7. The res ults were di scussed in context with possible applications in proclucing of plant material free of virus infections, within the scope of clonal selection, ancl in s toring germplasm.