Der Einfluß von Wachstumsinhibitoren auf die Langzeitlagerung von <i>in-vitro</i>-Kulturen der Rebe
DOI:
https://doi.org/10.5073/vitis.1987.26.57-64Keywords:
tissue culture, CCC, growth regulator, temperature, growth, shoot, root, callus, storageAbstract
- The effect of Cycocel (CCC) and Alar (B 995) on the growth of in vitro cultures of grapevine was tested under temperatures of +25, +8 and +3 °C. Both inhibitors were applied to the sterilized medium.
- Alar caused in rather low concentrations (100 ppm) such a strong growth inhibition that subculturing became difficult. Moreover, the once induced inhibition was maintained in the two subsequent passages after Alar application.
- CCC at concentrations of 500 to 1000 ppm reduced the length of the internodes of all cultivars tested to an extent of 30-40 % and the number of nodes/pla ntlet to 20-30 %, but delayed subculturing from 2-3 months to 5-7 months. An after-effect of CCC on subcultured plants was not observed.
- CCC-treated plantlets had sma ller and darker green leaves. Callus formation at the base of the explants was stimulatecl, whereas root growth was either unaffectecl or s lightly enhanced.
- CCC (750 ppm) prolonged vitality of the explants during a long- term storage at +8 °C.
- CCC-treated plantlets (750 ppm) survivecl after a storage period of 10 months at a recluced stor age t emperature of +3 °C without any loss of vitality, whereas untreated plantlets did not survive.
- The res ults were di scussed in context with possible applications in proclucing of plant material free of virus infections, within the scope of clonal selection, ancl in s toring germplasm.
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