Método bioquímico de determinación de la afinidad entre injerto y patrón en vid

  • A. Masa Vazquez
Keywords: affinity, biochemistry, protein


Biochemical method for determination of scion-rootstock affinity in grape

A biochemical method for the determination of affinity or incompatibility between scions and rootstocks in grape grafting is described. This method is based on the comparison of scions' and rootstocks' total proteins.
Total proteins of 5 Vitis vinifera L. cultivars (Airén, Bobal, Garnacha, Tempranillo, Viura) and 6 rootstocks (420-A, 41-B, 99-R, 110-R, 161-49, 196-17C) were extracted and analysed by polyacrylamide gel electrophoresis. From the REM (relative electrophoretic mobilities) of proteins the affinity indexes between scion and stock (KI-P) and stock and scion (KP-I) combinations were calculated. The results obtained were confronted with those advanced from cultural studies. The application of this biochemical method can allow to learn a priori which rootstocks may be compatible with a given cultivar.