In vitro propagation of Vitis: <p>The effects of organic substances on shoot multiplication</p>
DOI:
https://doi.org/10.5073/vitis.1985.24.106-118Keywords:
tissue culture, cytokinin, vitamin, amino acidAbstract
The effects of organic substances on shoot multiplication from subcultured shoots of the Vitis hybrid Remaily Seedless were investigated. Culture media contained the inorganic nutrients of MURASHIGE and SKOOG (1962).Benzylaminopurine (BAP) was most effective in promoting shoot multiplication at 5 μM. 60 % of the shoots had at least 3 nodes (1.5 cm total length), a size considered adequate for micropropagation.
The addition of adenine sulfate from 2.5 to 20 x 10 - 4 M depressed shoot multiplication when BAP was optimal.
Combinations of thiamine (2 and 4 μM) and inositol (50; 100; 500 μM) were tested. Shoot multiplication decreased with increasing inositol concentration and was higher with 4 μM of thiamine. Best multiplication was with 4 μM thiamine and 50 μM inositol and gave similar number of shoots per explant as obtained on our standard medium which contains in addition nicotinic acid and pyridoxine. However, twice _as many 3-node shoots were produced on the standard medium.
The amino acids arginine, aspartate, asparagine, glutamate, glutamine and tyrosine at 2.5-40 x 10 - 4 M had no effect on shoot multiplication.
With the culture conditions used in this research, an adequate set of organic constituents for shoot multiplication of grapevines is defined as 3 μM thiamine · HCl, 55.5 μM myo-inositol, 8 μM nicotinic acid, 5 μM pyridoxine · HCl and 5 μM benzylaminopurine. The addition of 4 μM of aspartate may be beneficial. It was found that optimization of vitamin concentration is important in shoot multiplication. The restricted Optimum cytokinin concentration for best shoot production suggests that its concentration may have to be adjusted for each cultivar.
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