Ultrastructure of grape leaf protoplasts in comparison with the source tissue
DOI:
https://doi.org/10.5073/vitis.1983.22.193-201Abstract
Based on their histochemical properties, protoplasts from grape leaf mesophyll were found to be viable and devoid of remnants of the cell wall. The results were confirmed by electron microscopy of sectioned and freeze-etched protoplast preparations. The only remarkable difference between cells in the intact tissue and the protoplasts was the occurrence of electron-dense globules in the cytoplasm of some individuals. These "osmiophilic bodies" are believed to be due to inevitable plasmolytic effects during wall removal and seem not to have any influence on protoplast CO2-assimilation, as has been demonstrated earlier. lt therefore appears that the vital structures remain intact and functional, in spite of the stress situation created by the isolation procedure.
Ultrastruktur von Rebenblatt-Protoplasten und Ausgangsgewebe - ein Vergleich
Anhand histochemischer Nachweise, welche das Weiterfunktionieren der Plasmamembran als physiologische Barriere nach dem Isolationsprozeß bestätigen, können Mesophyllprotoplasten von Vitis vinifera L. als lebensfähig betrachtet werden. Dieser Befund, sowie das vollständige Fehlen von Zellwandresten, wurde durch elektronenmikroskopische Untersuchungen an Ultradünnschnitten bzw. Gefrierätzproben von Protoplastenpräparaten erhärtet. Das vereinzelte Auftreten von elektronendichten, runden Gebilden (sog. osmiophilic bodies) im Cytoplasma ist vermutlich eine der plasmolysierenden Wirkung des Isolationsmediums zuzuschreibende Erscheinung. Wie früher gezeigt, hat aber die verwendete Isolationsmethodik keinen negativen Einfluß auf den photosynthetischen C-Stoffwechsel der Protoplasten. Daraus geht hervor, daß die lebenswichtigen Zellkomponenten, trotz der isolationsbedingten Streßsituation, keinen irreversiblen und damit nachweisbaren, strukturellen oder funktionellen Beeinträchtigungen unterworfen sind.
Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.