Morphological responses of shoot apices of grapevine cultured <i>in vitro</i> <p>Effects of cytokinins in routine subculturing</p>
DOI:
https://doi.org/10.5073/vitis.1982.21.293-298Abstract
Shoots derived from shoot apices of grapevine were repeatedly subcultured on nutrient media containing difterent concentrations of 6-benzylaminopurine (BAP) and zeatin riboside (ZR). Optimum shoot proliferation resulted with a combination of the cytokinins, each at 2 mg/l. Shoots produced with ZR only (10 mg/l) elongated rapidly, were slim and erect, with almost normal leaves. With BAP at 2 mg/l alone and in combination with low concentrations of ZR {2 mg/l) proliferated shoots were thicker, often branched, less elongated, curved and not uniform in size. Leaves were atypical. It was concluded that application of cytokinins should be useful in the production of large numbers of grapevine clones.
Die morphologischen Reaktionen in vitro kultivierter Rebentriebspitzen
Der Einfluß von Cytokininen bei aufeinanderfolgenden Subkulturen
Aus Rebentriebspitzen gewonnene Triebe wurden wiederholt auf Nährmedien mit unterschiedlichen Konzentrationen von 6-Benzylaminopurin (BAP) und Zeatinribosid (ZR) übertragen. Eine optimale Triebvermehrung wurde mit 2 mg BAP/l in Verbindung mit 2 mg ZR/1 erzielt. Ohne BAP, nur mit 10 mg ZR/l erzeugte Triebe wuchsen rasch in die Länge, waren schlank und aufrecht und besaßen nahezu normale Blätter. Mit 2 mg BAP/l, allein oder mit 2 mg ZR/1 kombiniert, entstanden unterschiedlich große, gestauchte, oftmals verzweigte Triebe von gekrümmtem Wuchs und mit atypischen Blättern. Cytokinine dürften ein wertvolles Hilfsmittel darstellen, um Rebenklone in großer Zahl zu erzeugen.
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