Organogenesis in internode explants of grapevines
DOI:
https://doi.org/10.5073/vitis.1981.20.218-227Abstract
Organogenesis was studied in cultured internodes of grapevine cultivars, hybrids and species. Adventitious root formation occurred with a wide range of media and genotypes. Adventitious buds were formed in explanrts grown in ag1tated liquid culture with NITscH medium supplemented with benzyladenine (1 μM) and 2,4-D (5 μM} or a mixture of 2,4-D and ß-naphthoxyacetic acid (5 μM). Bud formation occurred only in cultures derived from seedlings of Muscadinia rotundifolia, Gloryvine (Vitis vinifera L. x Vitis rupestris ScHEELE) and V. vinifera x Gloryvine, i.e. (Grenache x Cabernet Sauvignon) x Gloryvine and (Sumoll x Cabernet Sauvignon) x Gloryvine. Cuttings from mature vines of V. vinifera cultivars, or from clonal Gloryvine, gave explants which failed to produce buds. Results are discussed in relation to effects of composition of media, origin of explant, maturity of the mother plant and genotype.
Organogenese bei Internodienexplaotaten von Reben
Bei Kalluskulturen aus Internodien verschiedener Rebsorten, -kreuzungen und -arten wurde die Organogenese untersucht. Adventivwurzeln bildeten sich auf zahlreichen Nährmedien und bei vielen Genotypen. Adventivknospen entstanden an Explantaten in Schüttelkulturen auf einem flüssigen Medium nach NITSCH mit einem Zusatz von Benzyladenin (1 μM) und 2,4-D (5 μM) oder einem Gemisch von 2,4-D und ß-Naphthyloxyessigsäure (5 μM). Knospen bildeten sich nur in Kulturen aus Sämlingen von Muscadinia rotundifolia, Gloryvine (Vitis vinifera L. x Vitis rupestris SCHEELE) sowie V. vinifera x Gloryvine, nämlich (Grenache x Cabernet Sauvignon) x Gloryvine und (Sumoll x Cabernet Sauvignon) x Gloryvine. Aus ausgereiftem Holz von V. -vinifera-Sorten oder einem Gloryvine-Klon hergestellte Stecklinge ergaben Explantate, die keine Knospenbildung zeigten. Die Ergebnisse werden im Hinblick auf die Zusammensetzung der Nährmedien, den Ursprung des Explantats, den Reifezustand der Mutterpflanze und den Genotyp diskutiert.
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