Organogenesis in internode explants of grapevines

Authors

  • K. Rajasekaran Department of Agronomy and Horticultural Science, University of Sydney, N.S.W., Australia
  • M. G. Mullins Department of Agronomy and Horticultural Science, University of Sydney, N.S.W., Australia

DOI:

https://doi.org/10.5073/vitis.1981.20.218-227

Abstract

Organogenesis was studied in cultured internodes of grapevine cultivars, hybrids and species. Adventitious root formation occurred with a wide range of media and genotypes. Adventitious buds were formed in explanrts grown in ag1tated liquid culture with NITscH medium supplemented with benzyladenine (1 μM) and 2,4-D (5 μM} or a mixture of 2,4-D and ß-naphthoxyacetic acid (5 μM). Bud formation occurred only in cultures derived from seedlings of Muscadinia rotundifolia, Gloryvine (Vitis vinifera L. x Vitis rupestris ScHEELE) and V. vinifera x Gloryvine, i.e. (Grenache x Cabernet Sauvignon) x Gloryvine and (Sumoll x Cabernet Sauvignon) x Gloryvine. Cuttings from mature vines of V. vinifera cultivars, or from clonal Gloryvine, gave explants which failed to produce buds. Results are discussed in relation to effects of composition of media, origin of explant, maturity of the mother plant and genotype.

Organogenese bei Internodienexplaotaten von Reben

Bei Kalluskulturen aus Internodien verschiedener Rebsorten, -kreuzungen und -arten wurde die Organogenese untersucht. Adventivwurzeln bildeten sich auf zahlreichen Nährmedien und bei vielen Genotypen. Adventivknospen entstanden an Explantaten in Schüttelkulturen auf einem flüssigen Medium nach NITSCH mit einem Zusatz von Benzyladenin (1 μM) und 2,4-D (5 μM) oder einem Gemisch von 2,4-D und ß-Naphthyloxyessigsäure (5 μM). Knospen bildeten sich nur in Kulturen aus Sämlingen von Muscadinia rotundifolia, Gloryvine (Vitis vinifera L. x Vitis rupestris SCHEELE) sowie V. vinifera x Gloryvine, nämlich (Grenache x Cabernet Sauvignon) x Gloryvine und (Sumoll x Cabernet Sauvignon) x Gloryvine. Aus ausgereiftem Holz von V. -vinifera-Sorten oder einem Gloryvine-Klon hergestellte Stecklinge ergaben Explantate, die keine Knospenbildung zeigten. Die Ergebnisse werden im Hinblick auf die Zusammensetzung der Nährmedien, den Ursprung des Explantats, den Reifezustand der Mutterpflanze und den Genotyp diskutiert.

Downloads

Published

2016-06-22

Issue

Vol. 20 No. 3 (1981): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.