Cytokinin-binding protein in grape berries
DOI:
https://doi.org/10.5073/vitis.1980.19.216-225Abstract
A cytokinin-binding protein was isolated from grape berries (Vitis labruscana cv. Delaware) by affinity chromatography on benzyladenine-linked Sepharose 4B. The adsorbable protein was eluted with 50 mM NaOH from the BA-Sepharose column. This protein consisted of at least two glycoprotein components which exhibited high or low affinity to cytokinin and Concanavalin A, respectively.
Ein Cytokinin-bindendes Protein in Traubenbeeren
Aus Traubenbeeren der Sorte Delaware (Vitis labruscana) wurde mit Hilfe der Affinitätschromatographie an Benzyladenin- (BA-)gekoppelter Sepharose 4B ein Cytokinin-bindendes Protein isoliert. Das adsorbierte Protein wurde aus der BA-Sepharose-Säule mit 50 mM NaOH eluiert. Es besteht aus mindestens zwei Glykoproteinkomponenten, die gegenüber Cytokinin und Concanavalin A eine hohe bzw. niedrige Affinität aufweisen.
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