<i>In vitro</i> propagation of grapevine (<i>Vitis vinifera</i> L.) from fragmented shoot apices
DOI:
https://doi.org/10.5073/vitis.1978.17.335-340Abstract
A method is described for the in vitro propagation of grapevine from fragmented shoot apices, which has the potential to produce approximately 8000 plantlets from a single apex within 4 months. Apical cell clumps were grown in a liquid culture medium with cytokinin but in the absence of auxin. Transfer of the differentiated cell clumps to the same medium gelled with agar resulted in shoot masses which could be repeatedly subcultured. Excised shoots rooted readily on a hormone-free basal medium, and were successfully transferred to glasshouse conditions. This method has potential value in commercial clonal grapevine propagation.
In-vitro-Vermehrung der Rebe (Vitis vinifera L.) aus zerstückelten Triebspitzen
Eine Methode für die in-vitro-Vermehrung der Rebe aus zerstückelten Triebspitzen wird beschrieben. Zellklumpen der Triebspitze wurden auf einem flüssigen Medium, das Cytokinin, aber kein Auxin enthielt, kultiviert. Nach Übertragung der differenzierten Zellklumpen auf ein mit Agar verfestigtes, aber sonst gleich zusammengesetztes Medium entstanden Büschel von Trieben, die zerteilt und weiter vermehrt werden konnten. Explantierte Einzeltriebe bewurzelten sich auf einem hormonfreien Grundmedium rasch.
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