Ultrastructure of pollen of <i>Vitis vinifera</i> L. cv. "Picolit giallo" and its behaviour in experiments of self- and cross-pollination
DOI:
https://doi.org/10.5073/vitis.1976.15.73-81Abstract
The ultrastructure of the pollen and stigma of Vitis vinifera L. cv. "Picolit giallo" was studied by scanning and transmission electron microscopy, with the aim of finding out if they presented abnormalities which could account far the low procluctivity of this vine variety. The pollen protoplast has a normai aspect, but its wall is continuous and does not present furrows and germinative pores as pollen grains generally do. The stigma appeared normai. The presence of an uninterrupted wall most probably constitutes an obstacle to pollen germination and is likely to be one of the causes of the low fertilization incidence of "Picolit giallo". This hypothesis is supported also by the results of the experiments of self- and cross-pollination, because Picolit pollen always failed to germinate, even if put on stigmas of other vine varieties, while the pollen of these varieties germinated regularly when put on Picolit stigmas.
Die Feinstruktur der Pollenkörner von Vitis vinifera L. cv. Picolit giallo und ihr
Verhalten bei Versuchen zur Selbst- und Fremdbestäubung
Die Vitis-vinifera-Sorte Pìcolit giallo liefert nur geringe Erträge. Um hierfür verantwortliche Abnormitäten aufzufinden, wurde die Feinstruktur von Pollenkörnern und Narbe mit Hilfe der Raster- und Transmissionselektronenmikroskopie untersucht. Der Protoplast der Pollenkörner wirkt normal, aber die Pollenwand ist von kontinuierlicher Beschaffenheit und zeigt keine Furchen oder Keimporen wie bei typischen Pollenkörnern. Die Narbe macht einen normalen Eindruck. Sehr wahrscheinlich verhindert die nicht unterbrochene Pollenwand die Pollenkeimung und ist somit eine mögliche Ursache der geringen Fertilität von Picolit giallo. Diese Vermutung wird durch Versuche zur Selbst- und Fremdbestäubung gestützt, wobei Picolit-Pollen nie keimte, auch dann nicht, wenn er auf Narben anderer Rebensorten gebracht wurde; umgekehrt keimte der Pollen solcher Sorten regelmäßig, wenn er auf Picolit-Narben übertragen wurde.
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