Production of callus from protoplasts of cultured grape pericarp
DOI:
https://doi.org/10.5073/vitis.1975.14.177-180Abstract
Protoplasts were isolated from callus cultures of grape vine pericarp after an overnight incubation at 26 °c in 2% Cellulase Onozuka SS plus 1 % Macerozyme dissolved in a mixture of 0.14 molal KCl and 0.10 molal CaCl2 at pH 5.5. When cultured in a liquid medium with either 0.24 M sorbitol or 0.23 M sucrose as osmotic stabilizer, new cell walls were regenerated within the first few days. Cell division commenced by the sixth day of culture, and approximately 0.5% of the population continued dividing. Eventual transfer of the culture to the surface of medium solidified with agar resulted in callus formation.
Kallusbildung aus Protoplasten von kultiviertem Traubenperikarp
Eine Nährlösung wird beschrieben, in der Protoplasten, die aus Kalluskulturen von Traubenperikarp isoliert worden waren, neue Zellwände bildeten und Zellteilungen eingingen. Spätere Übertragung auf einen festen Nährboden ergab aktives Kalluswachstum.
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