Ermittlung der Kalium- und Calciumverteilung in Traubenachsen von <i>Vitis vinifera</i> mit Hilfe der Röntgenstrahlenmikroanalyse am Rasterelektronenmikroskop
DOI:
https://doi.org/10.5073/vitis.1975.14.190-197Abstract
Bei einem Limberger-Klon wurden Untersuchungen durchgeführt, welche Beziehungen zwischen der Kalium/Calcium-Relation und der Stiellähmeerkrankung aufzeigen. Hierfür wurde ein Verfahren mit dem Rasterelektronenmikroskop und einem Röntgenspektrometer angewandt. Es gestattet die Analyse kleinster Gewebekomplexe im Anfangsstadium der Krankheit. Untersucht wurden völlig gesunde und kranke Pflanzen - bei letzteren sowohl gesund erscheinende Stellen an Traubenstielen als auch schwach erkrankte Gewebe. Die Messungen erfolgten an der Stieloberfläche und an Querschnitten.
Alle Untersuchungen zeigen, daß mit Beginn der Stiellähmekrankheit der Kalium/Calcium-Quotient größer wird. Davon sind die Oberfläche, das Rindenparenchym und auch das Xylem betroffen. Die größte Disharmonie zwischen Kalium und Calcium besteht im großzelligen Rindenparenchym.
Determination of the K/Ca ratio in rachises of the vine clusters (Vitis vinifera L.) by an X-ray microanalyser with a scanning electron microscope
The present study was conducted on rachises of Vitis vinifera L. during the early phase of stalk necrosis disease ('Stiellähme').
Amounts of K and Ca were recorded by an energy dispersion X-ray analyser with a scanning electron microscope.
Samples were taken from healthy and diseased plants, the affected plants being divided into seemingly healthy and slightly necrotic tissues.
In the case of the healthy-looking tissue from diseased plants, the ratio of K/Ca is high when compared with healthy plants. This tendency is accentuated in tissues with visible symptoms in the early phase of stalk necrosis disease.
The imbalance between K and Ca was considerable, especially in the large parenchyma cells of the cortex and was less accentuated in the xylem and on the surface of the rachis.
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