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Originalarbeit-Kurzmitteilung

First record of the leafhopper Hishimonus hamatus Kuoh, 1976 (Hemiptera: Cicadellidae) in Germany

Erstnachweis der Zikade Hishimonus hamatus Kuoh, 1976 (Hemiptera: Cicadellidae) in Deutschland

Patrick Winterhagen
Affiliation
Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Institute of Plant Protection, Neustadt a. d. Weinstraße, Germany

Journal für Kulturpflanzen, 72 (12). S. 586–589, 2020, ISSN 1867-0911, DOI: 10.5073/JfK.2020.12.04, Verlag Eugen Ulmer KG, Stuttgart

Correspondence
Dr. Patrick Winterhagen, Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Institute of Plant Protection, Breitenweg 71, 67435 Neustadt a. d. Weinstraße, E-Mail: patrick.winterhagen@dlr.rlp.de
Accepted
30 September 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/deed.en).
Dies ist ein Open-Access-Artikel, der unter den Bedingungen der Creative Commons Namensnennung 4.0 International Lizenz (CC BY 4.0) zur Verfügung gestellt wird (https://creativecommons.org/licenses/by/4.0/deed.de).

Abstract

When monitoring leafhoppers for phytoplasma vector management in the grapevine growing regions of Rhineland-Palatinate, Germany, some individuals of Hishimonus hamatus were collected at Neustadt a. d. Weinstraße. Specimen were caught by net as well as with Yellow Sticky Traps placed in shrubs. The species was determined visually and verified by molecular analysis. This is the first record of H. hamatus in Germany and also north of the Alp mountains denoting that this species is further expanding its territory in Europe.

Key words: Hishimonus, leafhopper monitoring, barcoding, cytochrome oxidase I (COI), first record in Germany

Zusammenfassung

Während einer Erhebung in Weinbauregionen von Rheinland-Pfalz in Deutschland zum Vorkommen von Zika­den, die als Vektor für Phytoplasmen eine Rolle spielen, wurden bei Neustadt a. d. Weinstraße einige Individuen von Hishimonus hamatus gefangen. Die Tiere wurden mittels Handfang und Gelbtafel-Klebefallen an Sträuchern gesammelt. Die Bestimmung der Art erfolgte visuell und molekulargenetisch. Es ist der Erstnachweis von H. hamatus für Deutschland und nördlich der Alpen, was auf eine weitere Ausbreitung dieser Art in Europa hinweist.

Stichwörter: Hishimonus, Zikadenmonitoring, barcoding, cytochrome oxidase I (COI), Erstnachweis für Deutschland

Introduction

The leafhopper Hishimonus hamatus is originally native to the Asian continent and this species obviously was introduced as an alien leafhopper to Europe. It was previously recorded in Slovenia (Seljak, 2013), Switzerland (Trivellone et al., 2015), Corse, France (Albre & Gibernau, 2019), and Italy (Quaglino et al., 2019). This indicates that the species has successfully established populations in Southern parts of Europe.

Material and Methods

Insect material

For the annual leafhopper monitoring approximately 40 Yellow Sticky Traps (Neudorff, Emmerthal, Germany) were distributed in grapevine growing regions of Rhineland-Palatinate and placed in or close to vineyards from July to October; the traps were checked fortnightly. In addition, leafhoppers were sampled from vegetation by net.

Molecular analyses

Genomic DNA was extracted with TNES-buffer in a non-destructive way to maintain the specimen (Sambrook & Russell, 2001; Peccoud et al., 2013). PCR was performed with the PuReTaq Ready-To-Go PCR Kit (GE Healthcare, Chicago, USA). Universal barcoding primers targeting the cytochrome oxidase I (COI) gene and PCR cycling conditions were applied as previously described (Folmer et al., 1994; Hebert et al., 2003). Obtained amplicons were sent for bi-directional sequencing (Eurofins Genomics, Ebersberg, Germany) and resulting sequences were compared by alignment and phylogenic analysis using Clustal Omega and MEGA-X (Sievers et al., 2011; Kumar et al., 2018) software, respectively. To test if phytoplasma were present in H. hamatus, PCR with the general primers P1 and P7 was performed (Lorenz et al., 1995).

Results and discussion

In order to assess phytoplasma vectors in the grapevine growing areas during the leafhopper monitoring in Rhineland-Palatinate, Germany, six individuals of H. hamatus were collected at a house garden close to vineyards located at the outskirts of Neustadt a. d. Weinstraße from July to September 2020. Four adult individuals were caught with Yellow Sticky Traps placed in a forsythia (Forsythia × intermedia) shrub or by net on golden privet (Ligustrum ovalifolium 'Aureum') leaves. Furthermore, two nymphs were collected with a net from golden privet leaves. The nymphs were kept on Ligustrum leaves in captivity for further development and final molting to the adult stage (Fig. 1). Collected individuals were determined visually as H. hamatus, which was confirmed on the molecular level (identifier at BOLD system: GERNW001PW, RLP001–20 and GERNW002PW, RLP002–20) (Fig. 2). Compared to the reference nucleotide (nt) sequence CORSE069–19 (658 nt) from an individual from France available at BOLD system the obtained COI core sequences share 99% identity with 3 and 4 missmatches for RLP001–20 (670 nt) and RLP002–20 (644 nt), respectively.

Fig. 1. Hishimonus hamatus collected on Ligustrum in Ger­many. 
A) 4th and B) 5th instar nymphs, C) freshly molted and still pale imago next to exu­via, D) and E) fully co­lored imago. Scalebar = 1 mm.

Fig. 1. Hishimonus hamatus collected on Ligustrum in Ger­many. A) 4th and B) 5th instar nymphs, C) freshly molted and still pale imago next to exu­via, D) and E) fully co­lored imago. Scalebar = 1 mm.

Fig. 2. Phylogenic tree displays maximum likelihood alignment of translated COI core sequences from German indivi­duals and sequences from different Hishimo­nus species available in databases. 
The species names and the corre­sponding identifiers are indicated.

Fig. 2. Phylogenic tree displays maximum likelihood alignment of translated COI core sequences from German indivi­duals and sequences from different Hishimo­nus species available in databases. The species names and the corre­sponding identifiers are indicated.

The presented data reveal the first verified record of H. hamatus in Germany and north of the Alps and the finding of developing nymphs confirms that the species is successfully propagating. A further random search of the immediate surroundings did not yield in more individuals. However, additional findings that still need to be verified on species level are expected from other areas in southwest Germany (personal communication, H. Nickel). How and when H. hamatus reached Germany is unknown at present.

It is noted that H. hamatus is polyphagous and feeds on various woody plants (Seljak, 2013). Due to the limited insect material available, only a preliminary study could be performed. At a feeding test a 5th instar nymph immediately accepted a grapevine leaf without further probing and fed on that for several hours before switching to the in parallel offered Ligustrum leaves from which it was collected before. This suggests that H. hamatus may accept an even wider range of plant species as food source then previously described. Regarding phytosanitary issues, it would be important to know if H. hamatus can act as a vector for phytoplasmas. A potential risk exists that phytoplasmas present in European plants and which may be compatible to H. hamatus might be taken up and spread by this leafhopper. However, at present there is no proof that H. hamatus transmits phytoplasma and an initial PCR test for detecting different phytoplasmas in H. hamatus DNA extracts (n = 2) turned out to be negative (data not shown). To obtain reliable data, representative studies with an appropriate number of individuals and transmission experiments are necessary.

It should be considered that introduced alien leafhopper species are candidates to become a vector, as they may be able to pick up compatible native phytoplasma from host plants and spread those (Malembic-Maher et al., 2020). A famous example for an alien species acting as vector is the leafhopper Scaphoideus titanus (Deltocephalinae) from America that was introduced in Europe and spreads the Flavescence dorée phytoplasma in vineyards (Chuche & Thiéry, 2014). Further, it was experimentally demonstrated that Orientus ishidae (Deltocephalinae), a polyphagous species originally native to Asia accidentally introduced to Europe, can be infected with 16SrV-group phytoplasmas and it is able to transmit those to grapevine (Mehle et al., 2010; Lessio et al., 2016). A similar scenario as found for O. ishidae cannot be excluded for H. hamatus yet because the genus Hishimonus also belongs to the subfamily Deltocephalinae and the species H. phycitis and H. sellatus are reported to transmit phytoplasmas (Tanaka et al., 2000; Cha & Han, 2002; EFSA, 2017). Conclusively, the occurrence of H. hamatus and the expansion of its territory in Europe should be further observed to identify any risk for horticultural or native plants as early as possible and to take appropriate measures if necessary.

Acknowledgerments

Thanks to Herbert Nickel for information and discussion about a possible further occurrence of H. hamatus in Germany.

Conflict of interest

The author declares no conflict of interest.

References

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