Molekularbiologische Diagnostik von Pflanzenkrankkeiten
DOI:
https://doi.org/10.5073/jka.2014.446.009Abstract
In derAbteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche Schaderreger für private Kunden durchzuführen. Verwendet werden unterschiedliche PCR Techniken um Phytoplasmen, Bakterien, Pilze, Viren, Viroide, Insekten und Nematoden zu diagnostizieren. Die Vorteile der PCR Techniken werden dargestellt.
Stichwörter: PCR, RT-PCR, qPCR, RT-qPCR, DNA-Barcoding
Molecular diagnosis of plant diseases
The Department for Molecular Diagnostics of Plant Diseases of the Austrian Agency for Health and Food Safety (AGES) uses molecular diagnostic tools to support control and execution tasks regulated by law, and to test private plant samples for plant pathogens. Different PCR techniques are used to investigate for phytoplasma, bacteria, fungi, virus, viroids, insects and nematods. The advantages of molecular tools are summarized.
Keywords: PCR, RT-PCR, qPCR, RT-qPCR, DNA-barcoding
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