Einfluss von abiotischen Faktoren auf Wuchs und Scopolamin-Biosynthese in <em>Duboisia myoporoides</em>.
DOI:
https://doi.org/10.5073/jka.2014.446.011Abstract
Duboisia ist eine in Australien beheimatete Pflanze aus der Familie der Nachtschattengewächse, die Tropanalkaloide als sekundäre Pflanzeninhaltsstoffe enthält, deren quantitativ und ökonomisch bedeutsamstes Scopolamin ist.Um neue Erkenntnisse hinsichtlich des Einflusses abiotischer Faktoren auf Wuchs und Scopolamin-Biosynthese zu gewinnen, wurden Pflanzen der Art Duboisia myoporoides in Hydrokultur in Klimakammern unter streng kontrollierten Bedingungen angebaut (kalibrierte Einstellungen für Beleuchtungsdauer, Lichtintensität und Temperatur). Wichtige Messgrößen stellten dabei Biomasse und Alkaloidgehalt dar, letzterer gemessen mittels UHPLC und LC-MS.
Stichwörter: Duboisia, LC-MS, Scopolamin, Tropanalkaloide
Influence of abiotic factors on growth and biosynthesis of scopolamine in Duboisia myoporoides.
Duboisia, a native Australian plant belonging to the family of Solanaceae, contains tropane alkaloids as secondary plant components, thereof quantitatively as well as economically most important scopolamine. In order to obtain new findings regarding the effect of abiotic factors on biomass and biosynthesis of scopolamine, plants of the species Duboisia myoporoides were grown in climate chambers using hydroponics under strictly controlled conditions (calibrated settings regarding lighting period, light intensity and temperature). Essential measurement variables were biomass and alkaloid content, the latter analyzed via UHPLC and LC-MS.
Keywords: Duboisia, LC-MS, scopolamine, tropane alkaloids
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Published
2014-08-14
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Themenkreis C: Phytopathologie und sekundäre Pflanzeninhaltsstoffe
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