Untersuchung der Thymian-Kollektion aus der Bundeszentralen Ex situ- Genbank Gatersleben – Vergleich morphologischer, phytochemischer und molekularer Merkmale
DOI:
https://doi.org/10.5073/jka.2014.446.018Abstract
18 Akzessionen der Gaterslebener Thymian-Kollektion aus sieben verschiedenen Arten wurden mit einem standardisierten Boniturschema morphologisch beschrieben. Besonderes Augenmerk lag dabei auf der Anzahl der Drüsenhaare. Außerdem wurden durch Durchflusszytometrie die Genomgröße bestimmt und der Ploidiegrad ermittelt. Die Verwandtschaftsverhältnisse wurden durch ITS-Marker analysiert. Des Weiteren wurde die Zusammensetzung des ätherischen Öls mittels Gaschromatographie gekoppelt an Massenspektrometrie untersucht. Verschiedene Chemotypen konnten dabei gefunden werden. Alle erhobenen Daten werden verglichen und miteinander in Beziehung gesetzt.
Stichwörter: Ätherisches Öl, Chemotypen, Drüsenhaare, ITS-Analyse, Thymus spp.
Screening of the thyme collection of the federal ex situ genebank in Gatersleben – comparison of morphological, phytochemical and molecular data
18 accessions of the Gatersleben thyme collection from seven different species were characterized morphologically with a standardized descriptor. A special focus was on the number of secretory cells. Besides, the genome size was detected with flow cytometry in order to determine the number of chromosomes and the ploidy level. ITS markers were used to analyze the phylogenetic relationship. In addition, the essential oil compounds were studied with gas chromatography coupled with mass spectrometry. Within the 18 accessions different chemotypes could be found. All data will be compared and evaluated.
Keywords: Chemotypes, essential oil, ITS analysis, secretory cells, Thymus spp.
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