Molekulare Charakterisierung einer Wildgerstenintrogression mit dem Gelbverzwergungsresistenzgen <i>Ryd4<sup>Hb</sup></i> im Genom der Kulturgerste
Abstract
Zusammenfassung
Aus einer interspezifischen Kreuzung der Wintergerste Hordeum vulgare cv. 'Igri' mit der virusresistenten Wildgerste H. bulbosum wurden spaltende Kartierungspopulationen entwickelt, die eine 3HL-Introgression tragen, welche Resistenz gegenüber BYDV vermittelt. Die genetische Analyse einer BC2F4-Familie zeigte eine monohybride Aufspaltung der Resistenz mit dominanter Merkmalsausprägung. Es konnten bisher vier Gersten- Ankermarker und vier TC-Marker um das Resistenzgen Ryd4Hb kartiert werden. Zwei der aus den orthologen Bereichen des Reisgenoms abgeleiteten TC-Marker kosegregieren mit dem Resistenzgen Ryd4Hb.
Stichwörter: Hordeum vulgare, Hordeum bulbosum, BYDV, Resistenz, Selektionsmarker
Abstract
Interspecific crosses between winter barley H. vulgare cv. 'Igri' and a virus-resistant accession of the wild species H. bulbosum yielded mapping populations segregating with individuals resistant towards barley yellow dwarf virus. Genetic analysis of a BC2F4 family revealed a dominant inheritance of the resistance. GISH analysis indicated an Hb introgression on chromosome 3HL. Four 3HL barley anchor markers and four TC markers of the orthologous rice genome region, respectively, were mapped on either side of the resistance locus Ryd4Hb. Two of the TC markers cosegregated with the BYDV resistance.
Keywords: Hordeum vulgare, Hordeum bulbosum, BYDV, resistance, selection markers
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