Application of current in situ hybridization techniques for grape phylloxera (<i>Daktulosphaira vitifoliae</i>, Fitch) and grapevine (<i>Vitis</i> spp. L.)
DOI:
https://doi.org/10.5073/vitis.2008.47.113-116Keywords:
in situ hybridization, in situ PCR, grapevine, grape phylloxeraAbstract
In situ hybridization and in situ PCR directly localize specific DNA and RNA sequences in tissues. To exactly focus on the processes occurring on cell- or tissue level, in situ techniques can be efficiently employed. Recent advances in viticultural research in the fields of genomics, proteomics and metabolomics are likely to employ these techniques to link DNA- or mRNA sequence information to physiological traits and processes occurring in the grapevine. In this paper, we present a range of possibilities for in situ techniques that can be applied in grapevine research. Two examples covering in situ PCR of grapevine roots and in situ hybridization of grape phylloxera will be given for illustration. Moreover, key steps of the techniques are discussed, which may be helpful to researchers aiming to employ in situ hybridization or in situ PCR.
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