Effect of five antimicrobial peptides on the growth of <i>Agrobacterium tumefaciens, Escherichia coli</i> and <i>Xylella fastidiosa</i>
DOI:
https://doi.org/10.5073/vitis.2003.42.95-97Keywords:
cecropins, growth inhibition, lytic peptides, magainins, Pierce’s disease, Shiva-1Abstract
Five lytic peptides, cecropin A (CA), cecropin B (CB), magainin I (MI), magainin II (MII) and Shiva-1 (a synthetic analog of CB), were evaluated for their antimicrobial activity against Escherichia coli and two pathogenic bacteria of grape, Agrobacterium tumefaciens and Xylella fastidiosa. Peptides were tested at 0.1, 0.5, 1.0, 5.0 and 10.0 μM concentrations in liquid nutrient medium-based assay to determine their relative efficacy in inhibition bacterial growth. A 24-h incubation of E. coli with the respective lytic peptides revealed that both CA and CB exhibited the highest antibacterial activity and completely inhibiting bacterial growth at concentrations as low as 0.5 μM. In contrast, MI, MII and Shiva-1 had no inhibiting effect on E. coli at the tested concentrations. Both CA and CB at concentrations greater than 0.5 μM were highly effective in inhibiting the growth of Agrobacterium, whereas complete inhibition was observed only at MI, MII and Shiva-1 concentrations greater than 5 μM. Growth inhibition profiles of X. fastidiosa were similar to those of E. coli, although Shiva-1 at 10 μM showed a modest growth inhibition.
Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.