Evidence for the involvement of cysteine proteases in the regulation of methyl jasmonate-induced cell death in grapevine
DOI:
https://doi.org/10.5073/vitis.2002.41.115-121Keywords:
Vitis vinifera L., Limberger, caspases, apoptosis, protease inhibitorsAbstract
A new system to study programmed cell death (PCD) in plants is described. Grapevine (Vitis vinifera L., cv. Limberger) leaves and suspension cells were induced to undergo a form of cell death that mimics the hypersensitive response (HR) by treatment with a lipid-derived molecule, methyl jasmonate (MeJA). This chemical-induced cell death was accompanied by the characteristic features of apoptosis in animal and plant cells, such as typical changes in nuclear morphology, the fragmentation of the nucleus and protoplast collapse. Local and ectopic treatment of grapevine leaves with phenylmethylsulfonyl fluoride (PMSF), leupeptin, and especially with a specific inhibitor of cysteine proteases, E-64, inhibited MeJA-induced cysteine protease activity and blocked PCD triggered by 50 muM MeJA. These results indicate that proteolysis plays a crucial role in MeJA-induced apoptosis and that this type of PCD can be regulated by activity poised between the cysteine protease and the cysteine protease inhibitor.
Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.