Biological activity of the elicitor released from mycelium of a grapevine isolate of the necrotrophic fungus <i>Botrytis cinerea</i>

Authors

  • V. Repka
  • I. Fischerová
  • K. Šilhárová

DOI:

https://doi.org/10.5073/vitis.2001.40.205-212

Keywords:

Vitis vinifera L., cell death, defense response, gene expression, oxidative burst, PR proteins, runoff transcription, Western slot blot

Abstract

To obtain primary insight into the pathway(s) by which defense responses in grapevine (Vitis vinifera L.) are induced, suspension cultures of grapevine cells were treated with an elicitor released from the mycelium of the necrotrophic fungal pathogen Botrytis cinerea (PERs et FRIES). It induced a typical array of defense responses, including cell death accompanied by the production of H2O2 from the oxidative burst and accumulation of diverse groups of pathogenesis-related (PR) proteins and key enzymes of the general phenylpropanoid pathway, comprising phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS) and chalcone isomerase (CHI). Nuclear run-off experiments demonstrated that the fungal elicitor caused rapid transcriptional activation of genes encoding diverse defense-related products followed by a massive salicylic acid production.

 

Downloads

Published

2015-05-05

Issue

Vol. 40 No. 4 (2001): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.