Production of triploid grapes by <i>in ovulo</i> embryo culture

Authors

  • H. Yamashita
  • I. Shigehara
  • T. Haniuda

DOI:

https://doi.org/10.5073/vitis.1998.37.113-117

Keywords:

tissue culture, Vitis spp., table grape, breeding, embryo rescue, seedlessness, triploid

Abstract

Triploid grapes were produced by in ovulo embryo culture. Ovules were obtained from 5 crosses: Rosario Bianco (V. vinifera L. 2x) x Kyoho (V. vinifera x V. labrusca L. 4x), Katta Kourgan(V. vinifera L. 2x)x Kyoho, Kyoho x Rosario Bianco, Kyoho x Sekirei (V. vinifera L. 2x) and Kyoho x Rizamat (V. vinifera L. 2x). Most embryos were obtained from ovules cultured at veraison. The addition of indole-3-acetic acid (2.0 mg·l-1) and gibberellic acid (0.4 mg·l-1 to 1/2 MS (half strength macro-elements) medium promoted the formation and growth of embryos. Although only a few embryos germinated directly from ruptured ovules, embryos excised from cultured ovules germinated successfully by in vitro culture. This paper demonstrates that in ovulo embryo culture is expected to be an efficient method to produce triploid grapes.

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Published

2015-08-05

Issue

Vol. 37 No. 3 (1998): Vitis

Section

Article

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