Grapevine virus A and grapevine virus D are serologically distantly related

Authors

  • E. Choueiri
  • N. Abou-Ghanem
  • D. Bosca

DOI:

https://doi.org/10.5073/vitis.1997.36.39-41

Keywords:

grapevine, trichovirus, GVA, GVB, GVD, serology, ELISA, western blot, tissue blot

Abstract

Grapevine trichovirus A (GVA), B (GVB), and D (GVD) are not serologically related as ascertained by ELISA and IEM tests using polyclonal antisera A study to investigate in detail their serological relationships was carried out with a larger number of reagents, including monoclonal antibodies (MAbs), and serological techniques (ELISA, IEM, tissue blot, Western blot). The results show that (i) polyclonal antisera to GVA, GVB and GVD cross-reacted in Western blot with all antigens; (ii) one out of 4 MAbs to GVA (MAb PA3.B9) reacted in ELISA, Western blot and tissue blot with the homologous virus and GVD but not with GVB. It is concluded that GVA, GVB and GVD are serologically distantly related and that the single antigenic determinant common to GVA and GVD is likely to be a cryptotope.

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Published

2015-08-06

Issue

Vol. 36 No. 1 (1997): Vitis

Section

Article

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