Immunodetection of chitinase-like and beta-1,3-glucanase-like proteins secreted <i>in vitro</i> by embryogenic and non-embryogenic cells of grapevines
DOI:
https://doi.org/10.5073/vitis.1997.36.51-52Keywords:
grapevine somatic embryogenesis, chitinase, β-1, 3-glucanase, Western blottingAbstract
Research Note
Embryogenic and non-embryogenic cell lines were obtained from the rootstock 41B (Vitis vinifera cv. Chasselas x V. berlandieri). A few extracellular proteins from culture media of embryogenic and non-embryogenic grapevine cell lines during multiplication showed a positive reaction with anti-chitinase antibodies (embryogenic cell lines) and anti-β-1,3-glucanase antibodies (non-embryogenic cell lines).
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