Immunodetection of chitinase-like and beta-1,3-glucanase-like proteins secreted <i>in vitro</i> by embryogenic and non-embryogenic cells of grapevines

Authors

  • A. Deloire
  • E. Kraeva
  • M. C. Mauro
  • E. Bonnet
  • J. Bierne

DOI:

https://doi.org/10.5073/vitis.1997.36.51-52

Keywords:

grapevine somatic embryogenesis, chitinase, β-1, 3-glucanase, Western blotting

Abstract

Research Note

Embryogenic and non-embryogenic cell lines were obtained from the rootstock 41B (Vitis vinifera cv. Chasselas x V. berlandieri). A few extracellular proteins from culture media of embryogenic and non-embryogenic grapevine cell lines during multiplication showed a positive reaction with anti-chitinase antibodies (embryogenic cell lines) and anti-β-1,3-glucanase antibodies (non-embryogenic cell lines).

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Published

2015-08-06

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