Procyanodols ologomères des sarments de vigne, cépage Carignan

Authors

  • M. Boukharta
  • M. Girardin
  • M. Metche

DOI:

https://doi.org/10.5073/vitis.1994.33.5-10

Keywords:

procyanidin, polyphenol, Vitis vinifera, shoot, Carignan, identification

Abstract

Les procyanidols des sarments de vigne (cépage Carignan) ont été obtenus par extractions successives à l'aide du mélange acétone- eau puis d'acétate d'éthyle. La combinaison des méthodes de fractionnement sur colonnes de Sephadex LH-20 et de Fractagel TSK HW40 avec la chromatographie préparative sur couches minces de silice complétée par I'HPLC semi-préparative, ont permis de purifier onze procyanidols. A l'aide de méthodes chimiques, enzymatiques et spectrophotométriques, ont été établies les structures de six procyanidols dimères B1, B2, B3, B4, B7 et B2-3'-O-gallate, de deux procyanidols trimères C1 et [épicatéchine ( 4 ß->8) épicatéchine ( 4 ß->8) catechine] ainsi que celles de trois monomères (-)epicatechine-3-0-gallate, (-)épicatéchine et ( + )catéchine.

Oligomeric procyanidins of vine shoots of cultivar Carignan

Procyanidins from vine shoots were extracted using acetone - water and ethyl acetate. The purification of procyanidins were carried out by combination of column chromatography on Sephadex LH-20 and Fractogel TSK HW 40, preparative TLC on silicagel and HPLC. Two trimeric procyanidins C1 and [epicatechin (4ß->8) epicatechin (4ß->8) catechin], six dimeric procyanidins B1, B2, B3, B4, B7 and 3'-O-galloyl B2 along with 3-O-galloyl (-)epicatechin, (+)catechin and (-)epicatechin were identified on the basis of chromatographical and chemical evidences.

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Published

2015-08-19

Issue

Vol. 33 No. 1 (1994): Vitis

Section

Article

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