Isozyme pattern comparison between tissue-cultured grapevines and mother plants

  • R. Botta
  • R. Vallania
  • M. L. Miaja
  • G. Vergano
  • G. Me
Keywords: enzyme, protein, analysis, leaf, variety of vine, clone, tissue culture, ampelography

Abstract

  • Isozyme analysis is one of the means suitable to characterize clonally propagated cultivars. Isoelectric focusing was used to reveal differences in isozyme patterns between tissue-cultured plants and mother plants, for the cultivars Barbera, Queen of the Vineyards, Dolcetto and Delight. In cultivar Barbera both 2n and 4n plants were considered.
  • Leaf samples were collected from shoots grown on cuttings under controlled environmental conditions and from plants obtained by tissue culture. The buds used for tissue culture were taken from the same shooted cuttings. Leaf extracts were analyzed by isoelectric focusing considering the following isozymes: AcPH (acid phosphatase), GPI (glucose phosphate isomerase) and PGM (phosphoglucomutase).
  • The banding patterns of GPI and PGM showed differences among the cultivars, while for AcPH there seemed to be no differences among them in the pH range considered. There were no differences between isozyme patterns of the Barbera 2n and Barbera 4n.
  • The main difference between in vitro plants and mother plants was the amount of isozyme evaluated by densitometric measurements. In all the cultivars, the amount of isozymes for AcPH was higher in mother plants than in in vitro ones, while for PGM and GPI it was the opposite.
  • This can be due to the different environmental conditions affecting cellular metabolism.
Published
2015-10-27
Section
Section 1: Genetic resources, evaluation and screening