Inheritance of isoenzymes and soluble proteins in grape varieties and F<sub>1</sub> hybrids
Keywords:genetics, ampelography, protein, enzyme, analysis, variety of vine, hybrid, Hungary, shoot, callus, must, wine
AbstractThe aim or our experiments was to verify the existence of a genetically interpretable molecular polymorphism in several grape varieties and their F1 hybrids, which we can employ for genetical and ampelographical characterization. In addition, we also programmed the progress of investigation methods.
The authors present protein and enzyme analysis of two pairs of parents, Pearl of Csaba x S. V. 12375 and Saperavi x Blaufrankisch, and of ten other cultivars and several F1 progenies.
The best experimental results for genetic markers can be gained when shoot and callus samples are collected at the end of winter, in February, at the same time. Spring shoot collection is less effective because of high chlorophyll contents, and must and wine samples are less suitable due to their microbial contamination.
The esterase enzyme group gives well reproducible, characteristically differentiated patterns. The enzyme patterns of parental varieties typically segregate in individual progenies, hence they prove to be good markers.
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