Grapevine root transformation with <i>Agrobacterium rhizogenes</i>
AbstractGrapevine shoots were obtained from tissue cultures of cvs Barbera, Moscato bianco and Nebbiolo; their stems were wounded and inoculated with the following strains of Agrobacterium rhizogenes. A4, 8196, NCPP 2659, and 15834. Root production at the inoculation site was obtained in about 25% of the stems of all cultivars with all the bacterial strains, with the exception of NCPP 2659, which gave a lower root proliferation rate. Cultures of roots obtained after inoculation were established and their growth was enhanced by some media and by addition of cytokinins and auxins to the medium. Root cultures obtained after inoculation with the strain 8196 were genetically transformed, as shown by opine production. In contrast, opines were seldom detected in the root cultures obtained with the other bacterial strains. Light microscope observation showed that the cortex of transformed roots has more cell layers and a larger average cell size than in normal roots.
Copyright (c) 2015 I. Gribaudo, A. Schubert
This work is licensed under a Creative Commons Attribution 4.0 International License.
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.