High frequency regeneration from grapevine petioles: Extension to new genotypes
AbstractAuxins were found to enhance shoot organogenesis when used in a high cytokinin medium at low levels. This enhancement was genotype specific. In the presence of 10.0 µM BA, Vanessa regenerated best with the inclusion of 4.0 µM IAA-dl-aspartate, while Catawba regenerated best with 2.0 µM IAA-glycine in the medium. With Ravat 51, 14-15% of explants regenerated in the presence of either 2.0 µM IAA-glycine or 4.0 µM IAA-dl-aspartate, whereas there was no regeneration in the absence of auxin. Tests with 0.0-8.0 µM TDZ as a cytokinin source indicated that petiole explants of Vanessa regenerate best (10%) at 4.0 µM TDZ. Our results clearly show that high cytokinin media can be easily extended to petiole explants of other genotypes to facilitate shoot organogenesis and that low levels of auxins, especially IAA conjugates can enhance the level of regeneration.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.