Conservation of the genetic resources of <i>Vitis</i>
Keywords:gene resources, tissue culture, long-term storage, preculture, light condition, growth regulator, carbon dioxide
AbstractAs an alternative or supplement to field collections, a repository of 35 grapevine genotypes and 15 clones of cv. Optima was set up under minimal growth conditions. To prolong the storage period between subculturing to at least 12-18 months, some factors of long-term storage were optimized and the causes responsible for early senescence of sensitive genotypes were investigated
Besides indirect effects due to excision time, origin of the starting material and preculture, there is a direct influence of the light conditions on the survival rate of cultures. CCC application showed, in some cases, positive effects only after long-term storage.
An increase of carbon dioxide concentration during storage is considered to be responsible for the early death of some cultures.
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.