Meristem culture for clonal micropropagation of grapevines
Keywords:tissue culture, micro-propagation, growth, meristem, root, shoot, culture medium, vitamin, growth regulator
AbstractExperiments were carried out to determine the effects of MS medium concentrations (4/4 MS, 3/4 MS, 2/4 MS) combined with GA3 at 0.0, 0.1 and 0.5 mg/l, and vitamin formulations (MS, Morel, thiamine-inositol) on success of meristem culture and of 15 auxin (IBA) x cytokinin (BAP) combinations on shoot and root formation for clonal rnicropropagation of the wine grape cv. Kalecik Karasi and the rootstock cv. 41 B M.G.
Best results were obtained from standard MS mineral composition and vitamin formulation combined with 2.5 mg/l BAP and 0.5 mg/l GA3 for primary meristem cultures of both genotypes; 0.0 mg/l IBA x 1.0 mg/l BAP for shoot subculture and 1.0 mg/l IBA x 0.0 mg/l BAP for root subculture of Kalecik Karasi; 0.0 mg/l IBA x 1.0 mg/l BAP for shoot subculture and 5.0 mg/l IBA x 0.0 mg/l BAP for root subculture of 41 B, considering shoot, explant, root, callus and particularly entire plant formation in the cultures of both stages.
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