Meristem culture for clonal micropropagation of grapevines

Authors

  • H. Çelik
  • M. Batur

DOI:

https://doi.org/10.5073/vitis.1990.29.special-issue.455-462

Keywords:

tissue culture, micro-propagation, growth, meristem, root, shoot, culture medium, vitamin, growth regulator

Abstract

Experiments were carried out to determine the effects of MS medium concentrations (4/4 MS, 3/4 MS, 2/4 MS) combined with GA3 at 0.0, 0.1 and 0.5 mg/l, and vitamin formulations (MS, Morel, thiamine-inositol) on success of meristem culture and of 15 auxin (IBA) x cytokinin (BAP) combinations on shoot and root formation for clonal rnicropropagation of the wine grape cv. Kalecik Karasi and the rootstock cv. 41 B M.G.
Best results were obtained from standard MS mineral composition and vitamin formulation combined with 2.5 mg/l BAP and 0.5 mg/l GA3 for primary meristem cultures of both genotypes; 0.0 mg/l IBA x 1.0 mg/l BAP for shoot subculture and 1.0 mg/l IBA x 0.0 mg/l BAP for root subculture of Kalecik Karasi; 0.0 mg/l IBA x 1.0 mg/l BAP for shoot subculture and 5.0 mg/l IBA x 0.0 mg/l BAP for root subculture of 41 B, considering shoot, explant, root, callus and particularly entire plant formation in the cultures of both stages.

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Published

2015-10-27

Issue

Vol. 29 (1990): Vitis (Special Issue)

Section

Section 5: Tissue and cell culture

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