Powdery mildew responsive genes of resistant grapevine cultivar 'Regent'

Authors

  • L. J. Welter Federal University of Santa Catarina, Campus of Curitibanos 89520-000 Brazil
  • C. Tisch State Education and Research Center of Viticulture, Horticulture and Rural Development, Institute of Plant Protection, Breitenweg 71, 67435 Neustadt/Weinstraße
  • A. Kortekamp State Education and Research Center of Viticulture, Horticulture and Rural Development, Institute of Plant Protection Breitenweg 71, 67435 Neustadt/Weinstraße
  • R. Töpfer Julius Kuehn Institute – Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof 76833 Siebeldingen
  • E. Zyprian Julius Kuehn Institute – Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof 76833 Siebeldingen

DOI:

https://doi.org/10.5073/vitis.2017.56.181-188

Keywords:

Erysiphe necator, gene induction, transcription factors, ERF, MYB, WRKY

Abstract

The ascomycete Erysiphe necator causes powdery mildew disease of grapevine, a disastrous infection which is commonly defeated with multiple fungicide applications in viticulture. Breeding for natural resistance of quality grapes (Vitis vinifera) is thus a major aim of current efforts. The cultivar 'Regent' is resistant to powdery mildew due to an introgression from an American Vitis sp. resistance donor. To identify key regulatory elements in defense responses of 'Regent' we performed transcript analyses after challenging with E. necator inoculation in comparison with a susceptible grapevine. A set of genes selected from preliminary microarray hybridization results were investigated by RT-qPCR. The data indicate an important role of transcription factors MYB15, WRKY75, WRKY33, WRKY7, ethylene responsive transcription factors ERF2 and ERF5 as well as a CZF1/ZFAR transcripton factor in regulating the early defense when the fungus starts the interaction with its host by the formation of haustoria.

Downloads

Additional Files

Published

2017-11-06

Issue

Vol. 56 No. 4 (2017): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.