Powdery mildew responsive genes of resistant grapevine cultivar 'Regent'
Keywords:Erysiphe necator, gene induction, transcription factors, ERF, MYB, WRKY
AbstractThe ascomycete Erysiphe necator causes powdery mildew disease of grapevine, a disastrous infection which is commonly defeated with multiple fungicide applications in viticulture. Breeding for natural resistance of quality grapes (Vitis vinifera) is thus a major aim of current efforts. The cultivar 'Regent' is resistant to powdery mildew due to an introgression from an American Vitis sp. resistance donor. To identify key regulatory elements in defense responses of 'Regent' we performed transcript analyses after challenging with E. necator inoculation in comparison with a susceptible grapevine. A set of genes selected from preliminary microarray hybridization results were investigated by RT-qPCR. The data indicate an important role of transcription factors MYB15, WRKY75, WRKY33, WRKY7, ethylene responsive transcription factors ERF2 and ERF5 as well as a CZF1/ZFAR transcripton factor in regulating the early defense when the fungus starts the interaction with its host by the formation of haustoria.
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