The use of activated charcoal in grapevine tissue cultures

Authors

  • R. Oláh National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, Kecskemét, Hungary

DOI:

https://doi.org/10.5073/vitis.2017.56.161-171

Keywords:

embryo culture, in vitro culture, micropropagation, protoplast culture, tissue culture media, virus elimination, Vitis

Abstract

The microporous structure, the extremely large inner surface associated with adsorptive capacity and evidence of positive effects of activated charcoal (AC) in plant tissue culture media were published in several reviews (Pan and van Staden, 1998, Thomas 2008, Ahmadian et al. 2013). However a detailed overview on its application for grapevine is still lacking. In this review we present a comprehensive scale of results about the application of AC in different areas of grapevine tissue culture such as micropropagation, embryo rescue, somatic embryogenesis, gene transfer, protoplast culture, cryopreservation, viroid and virus elimination. These summarized data of positive results are aimed at stimulating a more extensive and effective use of AC as a powerful and beneficial component of grapevine tissue culture media.

Author Biography

R. Oláh, National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, Kecskemét, Hungary

senior research fellow

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Published

2017-10-09

Issue

Section

Review