The use of activated charcoal in grapevine tissue cultures
DOI:
https://doi.org/10.5073/vitis.2017.56.161-171Keywords:
embryo culture, in vitro culture, micropropagation, protoplast culture, tissue culture media, virus elimination, VitisAbstract
The microporous structure, the extremely large inner surface associated with adsorptive capacity and evidence of positive effects of activated charcoal (AC) in plant tissue culture media were published in several reviews (Pan and van Staden, 1998, Thomas 2008, Ahmadian et al. 2013). However a detailed overview on its application for grapevine is still lacking. In this review we present a comprehensive scale of results about the application of AC in different areas of grapevine tissue culture such as micropropagation, embryo rescue, somatic embryogenesis, gene transfer, protoplast culture, cryopreservation, viroid and virus elimination. These summarized data of positive results are aimed at stimulating a more extensive and effective use of AC as a powerful and beneficial component of grapevine tissue culture media.
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