Analysis of salinity tolerance of Vitis vinifera 'Thompson Seedless' transformed with AtNHX1

  • M. Venier Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Luján de Cuyo, Mendoza, Argentina
  • C. B. Aguero Department of Viticulture and Enology, University of California, Davis, USA
  • A. Bermejillo Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Luján de Cuyo, Mendoza, Argentina
  • M. F. Filippini Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Luján de Cuyo, Mendoza, Argentina
  • M. Hanana Department of Plant Sciences, University of California, Davis, USA
  • M. A. Walker Department of Viticulture and Enology, University of California, Davis, USA
  • E. Blumwald Department of Plant Sciences, University of California, Davis, USA
  • A. M. Dandekar Department of Plant Sciences, University of California, Davis, USA
Keywords: sodium chloride, potassium, grape, transgenic, hydroponics.

Abstract

Several transgenic plant species expressing AtNHX1, coding for a vacuolar Na+/H+ antiporter from Arabidopsis thaliana, have shown their ability to cope with salinity. The aim of this study was to analyze the response of Vitis vinifera cv. 'Thompson Seedless' transformed with AtNHX1 to salt stress, using soil substrate or hydroponic media, and to compare the response with untransformed 'Thompson Seedless' and allegedly tolerant 'Criolla' cultivars: 'Pedro Giménez' and 'Criolla Chica'. 'Thompson Seedless' embryogenic calli were transformed with Agrobacterium tumefaciens carrying AtNHX1 under the control of CaMV 35S promoter. Transgenic and untransformed plants were grown in a greenhouse under hydroponics or in pots with soil, and were subjected to increasing concentrations of sodium chloride (NaCl) up to 150 mM for a period of 7 weeks. Growth and toxicity symptoms were less affected in transgenics as compared to the untransformed grapevines, and transgenic lines had higher shoot length, leaf area and dry weights at the end of the experiment. Root concentrations of Na in transgenics were similar or lower than that observed in untransformed genotypes. Growth impairment and toxicity symptoms were observed in all genotypes under both conditions, but effects were more severe in plants growing with hydroponic culture. Potassium content and shoot to root dry weight ratio decreased with NaCl in hydroponics but not in pots. 'Criolla' cultivars grew less than the other genotypes, although 'Pedro Giménez' always exhibited highest shoot/root ratios.

Author Biographies

M. Venier, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Luján de Cuyo, Mendoza, Argentina

Jefe de Trabajos Practicos, Facultad de Ciencias Agrarias

C. B. Aguero, Department of Viticulture and Enology, University of California, Davis, USA

Associate Specialist, Department of Viticulture and Enology

A. Bermejillo, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Luján de Cuyo, Mendoza, Argentina

Profesora Adjunta, Facultad de Ciencias Agrarias

 

M. F. Filippini, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Luján de Cuyo, Mendoza, Argentina

Profesora Titular, Facultad de Ciencias Agrarias

M. Hanana, Department of Plant Sciences, University of California, Davis, USA

Researcher

M. A. Walker, Department of Viticulture and Enology, University of California, Davis, USA

Professor, Department of Viticulture and Enology

E. Blumwald, Department of Plant Sciences, University of California, Davis, USA

Professor, Department of Plant Sciences

A. M. Dandekar, Department of Plant Sciences, University of California, Davis, USA

Professor, Department of Plant Sciences

Published
2018-10-10
Section
Article