Complete chloroplast genome sequencing of Vitis vinifera subsp. sylvestris – wild ancestors of cultivated grapevines

Authors

  • Ia Pipia Institute of Molecular Genetics, Agricultural University of Georgia, Tibilisi, Georgia
  • Thierry Lacombe AGAP, Univ. Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France
  • Adam Kotorashvili National Center for Disease Control and Public Health (NCDC&PH), Tbilisi, Georgia
  • Nato Kotaria National Center for Disease Control and Public Health (NCDC&PH), Tbilisi, Georgia
  • Vazha Tabidze Institute of Molecular Genetics, Agricultural University of Georgia, Tibilisi, Georgia

DOI:

https://doi.org/10.5073/vitis.2023.62.193-201

Keywords:

Vitis vinifera subsp. sylvestris, Plastid DNA, Next-generation sequencing

Abstract

Wild grapevine – Vitis vinifera subsp. sylvestris – ancestors of cultivated grapevines are the main players in understanding the molecular bases of the grapevine domestication process. The goal of the presented research was to assess the genetic diversity of wild grapevine samples from several regions encompassing Europe (Spain, France, Germany, Hungary, Greece), the Mediterranean basin (Algeria, Tunisia, Morocco), and South Caucasus (Georgia), using a complete chloroplast DNA sequencing. The results suggest the existence of three different chloroplast DNA haplotypes, reflecting the geographical distribution of the analyzed samples. This study represents the first report focused on analysis of a wide range of wild grapevine samples (Vitis vinifera subsp. sylvestris), applying next-generation technologies, and tracing the grapevine ancestry.

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Published

2023-12-20

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