Antimycobacterial potential of green synthesized silver nano particles from selected Himalayan flora


  • Suman Mahmood Department of Biotechnology, University of Kotli, Pakistan
  • Sammyia Jannat Department of Biotechnology, University of Kotli, Pakistan
  • Asad Hussain Shah Department of Biotechnology, University of Kotli, Pakistan
  • Anila Fariq Department of Biotechnology, University of Kotli, Pakistan
  • Sajida Rasheed Department of Biotechnology, University of Kotli, Pakistan
  • Akhlaaq Wazeer SRL, DHQ Hospital Mirpur, Pakistan
  • Saleh H. Salmen Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
  • Mohammad Javed Ansari Department of Botany, Hindu College Moradabad, Mahatma Jyotibaphule Rohilkhand University Bareilly, India
  • Abdul Qayyum Department of Agronomy, The University of Haripur, Pakistan



Mycobacterium tuberculosis (Mtb) is a persistent threat to human life and a challenge to global public health. The pathogen’s antibiotic
resistance has become a serious problem, prompting the development of nanotechnology-based medicines to prevent multidrug resistance in microorganisms. The present study aimed to synthesize silver nanoparticles (AgNPs), using leaves extracts of Achillea millefolium, Artemisia campestris and Hedera nepalensis to analyze their antimycobacterial potential. The biosynthesized silver nanoparticlesnwere harvested and characterized through UV visible spectroscopy,nField Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray spectroscopy (EDX). The FESEM analysis showed, that selected plant-based silver nanoparticles were spherical in shape with a diameter ranging from 50 nm to 80 nm. Energy Dispersive X-ray spectroscopy revealed that constitute elements of silver nanoparticles are Ag, C, O, Cl and Ca. The biosynthesized AgNPs exhibited significant antibacterial potential against Mycobacterium tuberculosis. At a concentration of 50 μL Hedera nepalensis exhibited the highest growth inhibition at 97.33%, followed by Artemisia at 95%, whereas the percentage growth inhibition of Achillea millefolium at 50 μL concentration was 72.33% as compared to the Rifampicin (RIF) i.e., 40%. Fluorescence microscopy confirmed visible growth inhibition in both experimental and controlled cultures. Hedra nepalensis and Artemisia campestris showed promising potential to inhibit the growth of mycobacteria populations, indicating their potential for the development of novel nanomedicine to treat tuberculosis effectively.