Efficient determination of cysteine sulphoxides in <em>Allium</em> plants applying new biosensor and HPLC-MS² methods

Authors

  • K. Ziegert
  • W. Schütze
  • H. Schulz
  • M. Keusgen
  • F. Gun
  • E. R. J. Keller

Abstract

Cysteine sulphoxide (CSO) contents of 16 different accessions of garlic (Allium sativum L.) and 15 varieties of onion (Allium cepa L.) were measured using two different rapid analytical methods: a biosensoric approach and a newly developed HPLC-MS2 technique. Both methods allow quantification of naturally occurring cysteine sulphoxides present in Allium plants without time-consuming sample pretreatment such as derivatisation of amino acid derivatives prior to HPLC-separation. It has been found that the amount of alliin, which is the predominant CSO occurring in garlic, varies between 0.2 and 2.2 g/100 g dry matter. Contrary to that, isoalliin representing the main CSO in onion has been detected in significantly lower amounts (0.3 to 1.25 g/100 g dry matter).

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Published

2012-12-13

Issue

Vol. 80 No. 1 (2006): Journal of Applied Botany and Food Quality

Section

Article

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