Antioxidant and antihemolytic activities of methanol extract of <em>Hyssopus angustifolius</em>


  • Seyed Fazel Nabavi
  • Seyed Mohammad Nabavi
  • Claire Hellio
  • Heshmatollah Alinezhad
  • Mahboobeh Zare
  • Razieh Azimi
  • Robabeh Bahafar


This study was designed to evaluate antioxidant and antihemolytic activities of Hyssopus angustifolius flower, stem and leaf methanol extracts by employing various in vitro assays. The leaf extract showed the best activity in DPPH (63.2 ± 2.3 μg mL-1) and H2O2  (55.6 ± 2.6 μg mL-1) models compared to the other extracts. However, flower extract exhibited the highest Fe2+ chelating activity (131.4 ± 4.4 μg mL-1). The extracts exhibited good antioxidant activity in linoleic acid peroxidation and reducing power assays, but were not comparable to vitamin C. The stem (23.58 ± 0.7 μg mL-1) and leaf (26.21 ± 1 μg mL-1) extracts showed highest level of antihemolytic activity than the flower extract.