The <i>in vitro</i> antioxidative and cytotoxic effects of selected <i>Salvia</i> species water extracts

  • Ana Zoran Alimpic Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade
  • Nikola Kotur Laboratory for Molecular Biomedicine, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade
  • Biljana Stanković Laboratory for Molecular Biomedicine, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade
  • Petar D Marin Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade
  • Vlado Matevski Institute of Biology, Faculty of Natural sciences and mathematics, University "Ss. Cyril and Methodius"; Macedonian Academy of Sciences and Arts, Skopje
  • Najat Al Sheef Department of Biology, Faculty of Arts and Science, Al-Mergeb University, Al-Khoms
  • Sonja Duletić-Laušević Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade
Keywords: Salvia, water extracts, antioxidative, cytotoxic, phenolics, flavonoids

Abstract

The current paper presents antioxidant and cytotoxic activities and total phenolic and flavonoid content of the selected species of genus Salvia (Lamiaceae) growing wild in Macedonia (S. jurisicii Košanin, S. amplexicaulis Lam., S. ringens Sibth. & Sm.) and Libya (S. fruticosa Mill. and S. lanigera Poir.). Crude water extracts, obtained from aerial parts, were yielded from 6.50 to 14.32%. Total phenolic content was the highest in water extracts of S. amplexicaulis and S. ringens (226.30 and 189.01 mg GAE/g, respectively), while the flavonoids were the most abundant in S. jurisicii extract (32.36 mg QE/g). Antioxidant activities of extracts were measured using DPPH, ABTS and FRAP assays. S. amplexicaulis and S. ringens extracts showed the strongest antioxidant activity, measured using DPPH (14.21 and 23.44 μg/mL, respectively) and ABTS assays (2.91 and 2.42 mg AAE/g, respectively). In FRAP assay, S. amplexicaulis and S. fruticosa extracts exhibited strongest activity (1406.73 and 1191.51 µmol Fe(II)/g). Water extract of S. amplexicaulis and S. ringens performed the strongest cytotoxic activity against K562 cells (151.07 and 173.06 μg/mL, respectively). Based on these findings, it can be concluded that S. amplexicaulis and S. ringens water extracts could be considered as possible source of antioxidant and cytotoxic agents.

Published
2015-05-05