Genetic characterization of banana clones grown in Turkey based on nuclear DNA content and SRAP markers

  • Hasan Pinar Alata Horticultural Research Station, Erdemli, Mersin-Turkey
  • Mustafa Unlu Alata Horticultural Research Station, Erdemli, Mersin-Turkey
  • Mustafa Bircan Alata Horticultural Research Station, Erdemli, Mersin-Turkey
  • Filiz Baysal Alata Horticultural Research Station, Erdemli, Mersin-Turkey
  • Gulsemin Savas Tuna Department of Field Crops, Faculty of Agriculture, Namik Kemal University, Tekirdag-Turkey
  • Metin Tuna Department of Field Crops, Faculty of Agriculture, Namik Kemal University, Tekirdag-Turkey
  • Sezai Ercisli Atatürk University
Keywords: Musa, banana, molecular characterization, flow cytometry

Abstract

This study was conducted to investigate the genetic relationships among banana clones grown in Turkey based on their nuclear DNA contents and SRAP markers. Four banana clones including 'Dwarf Cavendish', 'Grand Nain', 'Azman' and local 'Erdemli' were used as plant material. Nuclear DNA content of the banana cultivars estimated by flow cytometer and varied between 1.766 pg ('Erdemli') and 2.028 pg ('Grand Nain'). 'Azman' and 'Dwarf Cavendish' was similar nuclear DNA content. Genetic similarities of 4 banana clones were between 0.63-0.91 based on SRAP molecular marker. The local  'Erdemli' banana clone was the most distinct from the others. In conclusion, there is a high level of genetic variation among the banana clones grown in Turkey and the local clone 'Erdemli' is the most distinct one. This study showed that nuclear DNA content analysis together with molecular markers can be useful to assess the relationships among banana clones.

Author Biography

Sezai Ercisli, Atatürk University
Horticulture
Published
2015-10-14