Sequence-based unique DNA marker for simultaneous genotyping of all three β-conglycinin subunit genes in soybean

  • Kiwoung Yang Sunchon National University
  • Senthil Kumar Thamilarasan Department of Horticulture, Sunchon National University
  • Jong-Min Ko Department of Functional Crop, NICS, RDA
  • Jungmin Ha Institute for Plant Breeding, Genetics and Genomics, University of Georgia
  • In-Youl Baek
  • Yeong Hoon Lee
  • Jong-Koo Kang Sunchon National University
  • Mi-Young Chung Department of Agricultural Education, Sunchon National University
  • Ill-sup Nou Sunchon National University
Keywords: Soybean, 3-subunit of β-conglycinin, molecular marker, MAS, Mutant soybean

Abstract

Soybean (Glycine max [L.] Merr.) seed is about 40% protein, most of which is accounted for by the major storage proteins β-conglycinin (7S globulin) and glycinin (11S globulin). β-conglycinin, which consists of three subunits, α, α', and β, is the main allergen in soybean. Accordingly, elimination of β-conglycinin from seed is one of the goals of soybean breeding programs. Soybean accessions PI 200485 and CS 1150 lack the α'- and α-subunits of β-conglycinin, respectively. In this study, we developed a 3-betacon marker that could simultaneously identify genotypes for the three subunit genes of β-conglycinin. Interestingly, the three subunit genes were amplified with three distinct bands representing varied amplicon sizes. Mutant alleles could be identified in progeny of a three-way cross, and the corresponding α- and α'-subunit proteins were clearly absent in each mutant accession. 3-betacon marker will be highly useful for soybean breeding programs to develop soybean cultivars with decreased β-conglycinin contents.

 

The online version of this article (doi: 10.5073/JABFQ.2015.088.041) contains supplementary files.

Published
2015-11-16