Effect of nitric oxide (NO) on the induction of callus and antioxidant capacity of Hyoscyamus niger under in vitro salt stress
DOI:
https://doi.org/10.5073/JABFQ.2018.091.004Abstract
Generation of reactive oxygen species (ROS) under salt stress can cause oxidative damage. Nitric oxide (NO) is considered as a functional molecule in alleviating the oxidative damage of salinity to plants through modulating antioxidant metabolism. In the present study, effects of sodium nitroprusside (SNP), a NO donor, on induction of callus and seed germination, and antioxidant capacity of Hyoscyamus niger seedlings were studied under 0, 50, 100 and 150 mM NaCl stress. NO stimulated the germination of NaCl-treated seeds. NaCl treatment significantly induced accumulation of H2O2 and thiobarbituric acid-reactive substances (TBARS) in Hyoscyamus niger, and application of 100 μM SNP stimulated ROS-scavenging enzymes and increased scavenging activity (DPPH), hydroxyl radical (OH•) scavenging activity and chelating activity of ferrous ions, resulting in lower lipid peroxidation induced by NaCl stress. Therefore, it can be concluded that the increased antioxidant capacity by NO might be responsible for its function in alleviating the inhibition of Hyoscyamus niger growth and cell damage by salt stress. Also the effect of SNP on the induction of callus of Hyoscyamus niger was investigated. Callus fresh weight increased significantly in the combined effect of 50 μM SNP with other plant growth regulators (PGR) compared to control. It is evident that SNP has a direct effect on the induction of callus by interacting with cytokinins and auxins.
Downloads
Published
Issue
Section
License
From Volume 92 (2019) on, the content of the journal is licensed under the Creative Commons Attribution 4.0 License. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes are labelled.
The copyright of the published work remains with the authors. If you want to use published content beyond what the CC-BY license permits, please contact the corresponding author, whose contact information can be found on the last page of the respective article. In case you want to reproduce content from older issues (before CC BY applied), please contact the corresponding author to ask for permission.