Molecular cloning and functional characterization of the flavonoid 3′-hydroxylase gene from Rubus coreanus Miquel

  • Seung Hee Eom Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea
  • Jae-Yean Kim Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701
  • Tae Kyung Hyun Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644

Abstract

Rubus coreanus Miquel is a Korean black raspberry used in folk medicine and functional foods. To investigate the biosynthesis pathway of anthocyanin in R. coreanus Miquel, the complete coding sequence of flavonoid 3′-hydroxylase (F3′H), designated as RcMF3′H1, was cloned for the first time using the Korean black raspberry transcriptome library. The deduced amino acid sequence of RcMF3′H1 contained the proline-rich “hinge” region, P450 consensus heme-binding domain, and F3′H-specific motifs. Phylogenetic analysis revealed that RcMF3′H1 was clustered into the same subgroup as other plant F3′Hs. In addition, expression analysis by quantitative real-time PCR revealed the involvement of RcMF3′H1 in methyl jasmonate-mediated anthocyanin biosynthesis. Furthermore, the ability of the RcMF3′H1 gene to complement the Arabidopsis transparent testa 7-1 mutant suggested that RcMF3′H1 encodes the functional F3′H enzyme involved in anthocyanin biosynthesis. Taken together, the cloning and molecular characterization of RcMF3′H1 will facilitate a better insight into the anthocyanin biosynthesis pathway in R. coreanus Miquel.

Published
2018-03-07