HPLC-PDA-ESI-MSn profiling of polyphenolics in different parts of Capparis spinosa and Capparis decidua as function of harvesting seasons

  • Tehseen Gull Department of Chemistry, University of Central Punjab, Lahore, Multan Campus, Multan, Pakistan
  • Bushra Sultana Department of Chemistry, University of Agriculture Faisalabad, Pakistan
  • Farooq Anwar Department of Chemistry, University of Sargodha, Sargodha, Pakistan
  • Wasif Nouman Department of Forestry & Range Management, Bahauddin Zakariya University, Multan
  • Eduardo Augusto dos Santos Rosa Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Universidade de Trás os Montes e Alto Douro (UTAD). Quinta de Prados, 5000-801 Vila Real (Portugal)
  • Raúl Domínguez-Perles Centro de Edafología y Biología Aplicada del Segura, National Council for Scientific Research (CEBAS-CSIC) Univeristy Campus of Espinardo, Edif. 25. 30100 Espinardo, Spain

Abstract

HPLC-PDA-ESI-MSn analysis of different parts such as stem bark, shoot, flower, fruit and root of Capparis spinosa (C. spinosa) and Capparis decidua (C. decidua), collected in rainy and dry seasons from the Cholistan desert of Pakistan, depicted the occurrence of a wide array of phenolics with quercetin, apigenin and kaempferol derivatives along with dicaffeoylquinic acid, caffeoylquinic acid and feruloylquinic acid as the main compounds. Kaempferol-3-glucoside (28.02-167.21 μg g-1dw) was found to be the principal component in all tested parts of both species while dicaffeoylquinic acid was detected only in the flowers and roots. The roots exhibited maximum contents of flavonoids and hydroxycinnamic acid derivatives. The harvesting period significantly (p<0.05) affected the concentration of phenolics wherein the samples collected in rainy season offered greater levels of phenolics than their counterpart. The roots and fruits of both species were found to be rich sources of phenolics. The findings of this research suggest the harvesting of the selected wild Capparis species in rainy season to maximize their antioxidant and nutraceutical benefits.

Published
2019-03-19