Rapid clonal propagation and valepotriates accumulation in in vitro cultures of Valeriana jatamansi Jones, a high-value medicinal plant
Valeriana jatamansi is well known for its medicinal and ethnobotanical values. An efficient and rapid in vitro propagation system for V. jatamansi is presented. The shoot bud explants from V. jatamansi plants were cultured on Murashige and Skoog (MS) media supplemented with different concentrations of plant growth regulators (PGR’s). MS medium supplemented with 2 mg/L of benzyl amino purine (BAP) produced shoot bud regeneration. However, the proliferation rate was slow with fewer shoots. The nodal segments were excised from the in vitro plants raised on BAP (2 mg/L) and multiplied by supplementing MS medium with different PGR’s at different concentrations. Among the tested growth regulators, supplementation of 10% coconut water resulted in maximum shoot length (6 cm), shoot number (13.0), root length (7.5 cm) and root numbers (19.6). The genetic fidelity of the in vitro raised plants was confirmed by analysis with RAPD and ISSR markers. GC-MS profiling of the root extract from in vitro raised plant revealed the presence of 21 compounds including valeric acid which is commercially and pharmaceutically important. The protocol developed here will be useful in the future towards large scale commercial production of valepotriates from V. jatamansi.
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