Optimization of ultrasound-assisted enzymatic hydrolysis extraction of tea polyphenols from green tea and their antioxidant activities
DOI:
https://doi.org/10.5073/JABFQ.2021.094.017Abstract
Production of natural extracts requires suitable processing conditions to facilitate the accumulation and preservation of bioactive ingredients. This study aimed to optimize the conditions for extracting tea polyphenols (TPs) from green tea using ultrasound-assisted compound enzymatic extraction (UACEE) technology with response surface methodology (RSM), based on a three-level, four-variable central composite rotatable design (CCRD). Extracted TPs yields were in the range of 16.48% to 28.77%; the experimental results were fitted to a second-order quadratic polynomial model and showed a good fit to the proposed model (R2 > 0.90). Compared with other ex-traction methods, UACEE exhibited significant advantages in the TPs extraction rate and preservation of catechins composition. The antioxidant activities of these extracts were also analyzed using reducing power and DPPH radical scavenging activity; all extracts showed excellent antioxidant activity in a dose-dependent manner, and UACEE extracts showed the strongest antioxidant activity in vitro.
Downloads
Published
Issue
Section
License
Copyright (c) 2021 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
From Volume 92 (2019) on, the content of the journal is licensed under the Creative Commons Attribution 4.0 License. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes are labelled.
The copyright of the published work remains with the authors. If you want to use published content beyond what the CC-BY license permits, please contact the corresponding author, whose contact information can be found on the last page of the respective article. In case you want to reproduce content from older issues (before CC BY applied), please contact the corresponding author to ask for permission.