Kalanchoe fedtschenkoi R. Hamet & H. Perrier, a non-conventional food plant in Brazil: HPLC-DAD-ESI-MS/MS profile and leaf histochemical location of flavonoids

Authors

  • Jamile Marques Casanova Laboratório de Anatomia Vegetal, Departamento de Botânica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Brazil https://orcid.org/0000-0002-0475-9212
  • Luana Beatriz dos Santos Nascimento Laboratório de Anatomia Vegetal, Departamento de Botânica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Brazil https://orcid.org/0000-0002-1827-1672
  • Livia Marques Casanova Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil https://orcid.org/0000-0002-0697-1575
  • Silvana Daflón Castricini Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil
  • Jacqueline Elis Elis de Souza Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil
  • Raíssa Mara Kao Yien Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil https://orcid.org/0000-0001-5203-514X
  • Sônia Soares Costa Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil
  • Eliana Schwartz Tavares Laboratório de Anatomia Vegetal, Departamento de Botânica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Brazil https://orcid.org/0000-0001-9668-2330

DOI:

https://doi.org/10.5073/JABFQ.2022.095.020

Abstract

Kalanchoe fedtschenkoi (Crassulaceae) is a non-commercial food species in Brazil, whose leaves are used in several aqueous preparations. Their morphology and potential nutraceuticals (e.g., antioxidant phenolics) have been poorly studied. Anatomical features are useful for the correct identification of plants, avoiding their misuse. We describe the anatomy and tissue flavonoid location of K. fedtschenkoi leaves, as well as the phenolic composition and the antioxidant activity of their aqueous extracts. The succulent leaves have a unistratified epidermis with anisocytic or helicocytic stomata, collateral vascular bundles, and phenolic idioblasts throughout the leaf blade. NP-reagent tests suggested the presence of kaempferol derivatives (yellow fluorescence) in the regular epidermis cells, subsidiary cells, and leaf mesophyll. An orange color, assigned to quercetin derivatives, was detected in stomata guard-cells and below the epidermis. The HPLC-DAD-ESI-MS/MS evidenced the presence of
flavonoids, mostly kaempferol glycosides (especially sagittatin A), some of them firstly described as constituents of K. fedtschenkoi. The extracts also showed high antioxidant activity. We report, for the first time, the complete anatomical description of K. fedtschenkoi leaves, the tissue location of flavonoids, and the flavonoid composition of the extracts. The high flavonoid content and antioxidant activity of K. fedtschenkoi leaves can be an advantage for their consumption as a food, in addition to being a possible source of nutraceuticals.

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Published

2022-11-14

Issue

Vol. 95 (2022): Journal of Applied Botany and Food Quality

Section

Plant analysis

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Copyright (c) 2022 The Author(s)

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