Ultrasound-assisted extraction and (U)HPLC-DAD-ESI-MSn analysis of phenolic compounds from black chokeberries cultivated in Turkey

Authors

  • Aysun Öztürk Department of Food Technology, Atatürk Horticultural Central Research Institute, Turkey
  • Christof B. Steingass Department of Beverage Research, Chair Analysis and Technology of Plant-based Foods, Geisenheim University, Germany https://orcid.org/0000-0001-8269-4525
  • Ralf Schweiggert Department of Beverage Research, Chair Analysis and Technology of Plant-based Foods, Geisenheim University, Germany
  • Reinhold Carle Altenriet, Germany
  • Oktay Yemiş Department of Food Engineering, Faculty of Engineering, Sakarya University, Turkey https://orcid.org/0000-0002-7461-5185
  • Sevcan Erşan Department of Chemical and Biomolecular Engineering, University of California, United States https://orcid.org/0000-0002-5334-1955

DOI:

https://doi.org/10.5073/JABFQ.2023.096.003

Abstract

Phenolic compounds from two black chokeberry cultivars 'Viking' and 'Nero' grown in Turkey were analyzed by high performance liquid chromatography-diode array detection-electrospray ionization-multistage mass spectrometry (HPLC-DAD-ESI-MSn). In the first step, five different solvents were compared to efficiently isolate phenolic compounds by ultrasound-assisted extraction. Three sequential extraction cycles using methanol/formic acid (95:5, v/v) sufficed for exhaustive extraction of anthocyanins, hydroxycinnamic acid derivatives, and flavonol glycosides from black chokeberry within merely 60 sec. A total of four cyanidin glycosides, two hydroxycinnamic acids, and five quercetin mono- and diglycosides were detected in both cultivars. Total anthocyanins (425-438 mg/100 g of fresh weight, FW), hydroxycinnamic acids (173-179 mg/100 g of FW), and flavonols (37 mg/100 g of FW) were determined in a similar range for both cultivars. Complementary, a rapid ultra-high performance liquid chromatography (UHPLC)-DAD method was developed, permitting a high throughput screening of chokeberry phenolics. The established methods were validated considering extraction recoveries, intra- and inter-day repeatability, calibration linearity, limit of detection (LOD), and limit of quantitation (LOQ). UHPLC provided a 2.3 times faster compound separation (30 min) and less solvent consumption than HPLC (68 min).

Downloads

Published

2023-03-28

Issue

Vol. 96 (2023): Journal of Applied Botany and Food Quality

Section

Plant analysis

License

Copyright (c) 2023 The Author(s)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

From Volume 92 (2019) on, the content of the journal is licensed under the Creative Commons Attribution 4.0 License. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes are labelled.

The copyright of the published work remains with the authors. If you want to use published content beyond what the CC-BY license permits, please contact the corresponding author, whose contact information can be found on the last page of the respective article. In case you want to reproduce content from older issues (before CC BY applied), please contact the corresponding author to ask for permission.