<em>In vitro</em> multiplication of <em>Lepidium meyenii</em> Walp.

Authors

  • H. Polzerová
  • M. Greplová
  • J. Frček

Abstract

The multiplication protocol of the medicinal plant Lepidium meyenii was established through axillary bud proliferation. Multiplication was initiated on modified MS supplemented with a combination of zeatin (2 μM) and gibberellic acid 3 (0.3 μM). The average number of proliferated shoots was 5.3 per segment. Shoots were rooted on modified MS medium with 9.8 μM of indole-3-butyric acid and 3 μM of a-naphthalene acetic acid. In vivo rooting was also successful and 94 % regenerated plantlets were acclimatized.

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Published

2012-03-05