Tolerance of transgenic Arabidopsis thaliana overexpressing apple MdAGO4.1 gene to drought and salt stress
The regulatory role of apple MdAGO4.1 gene in plant drought and salt resistance is unclear. In this study, transgenic A. thaliana in which the apple MdAGO4.1 gene was over-expressed was used to analyze the regulatory effects of the MdAGO4.1 gene on plant drought and salt resistance, to verify the function of the apple MdAGO4.1 gene. The seed germination rate, seedling fresh weight and root length of transgenic Arabidopsis strains in MS medium containing different concentrations of NaCl and mannitol were better than those of the wild type. The transgenic A. thaliana seedlings were more resistant to drought than wild type under drought stress. The transgenic strains were less affected by salt stress than the
wild type. Exposure to drought and salt stress reduced the relative elektrolyte leakage, malondialdehyde (MDA), superoxide anion (O2-), and hydrogen peroxide (H2O2) levels of the transgenic strain significantly compared with the levels in the wild type. The levels of proline, protective enzyme activities, and the expression of genes related to drought and salt stress resistance were significantly higher than those of the wild type. These results indicate that MdAGO4.1 overexpression improved drought and salt tolerance in transgenic Arabidopsis. This study can provide a theoretical basis for future research on stress tolerance mechanisms and breeding new varieties of fruit trees resistant to drought and salt.
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